A study was carried out in the Department of Seed Science and Technology, College of Agriculture, Raichur, to assess the response of seed quality of ajwain (Trachyspermum ammi L.) to varieties and spacing. The seed was harvested from a split plot field experiment conducted during Rabi 2025-26, in which three spacings (S1: 30 cm × 20 cm, S2: 50 cm × 20 cm and S3: 60 cm × 20 cm) were the main plots and four varieties (AA-01-19, Kurnool local, Bidar local and Gujarat Ajwain-1) were the sub-plots. Seed germination, shoot length, root length, seedling dry weight and seedling vigour indices were recorded. Seed from the wider spacing (S3) recorded the highest germination (90.91%), shoot length (6.08 cm), root length (9.95 cm), seedling dry weight (9.02 mg), SVI-I (1457) and SVI-II (820), while the closer spacing (S1) recorded the lowest values for all of them. Among the varieties, Gujarat Ajwain-1 produced the best quality seed, with 90.72% germination, SVI-I of 1463 and SVI-II of 808 andBidar local was the poorest. It is concluded that seed of Gujarat Ajwain-1 produced at 60 cm × 20 cm spacing is the most suitable for obtaining high quality seed in ajwain.