Field experiments were conducted during kharif 2024 and 2025 at College of Agriculture, Bheemarayanagudi (UAS, Raichur) to assess the effect of biochar and FYM on the root and shoot bioconcentration factors (BCFr, BCFs) and translocation factor (TF) of nutrients in maize (Zea mays L.). The experiment was laid out in a randomized complete block design with eleven treatments and three replications. The results revealed that the absolute control (T11) recorded the highest BCFs and TF for N, P, K and micronutrients, while T4 (RDF + FYM @ 5.0 t ha⁻¹ + biochar @ 5.0 t ha⁻¹) recorded comparatively lower values for most of the parameters. As BCF is the ratio of plant to soil nutrient concentration, the high values in T11 reflects depletion of soil-available nutrients and thus low plant nutrition. On the other hand, the lower values under T4 indicate that biochar and FYM retained nutrients in the soil and released them gradually in line with the crop demand. Further, BCF and TF should therefore be interpreted together with absolute nutrient uptake and soil fertility status. The experimental data also revealed biochar as the most effective amendment when it is applied in combination with FYM and inorganic fertilizers.