The present investigation was undertaken to identify the best organic and inorganic seed priming treatment for improving seed quality in ajwain (Trachyspermum ammi L.) variety AA-01-19 under laboratory conditions. The experiment comprised fourteen treatments, viz., unprimed control, hydropriming, humic acid (2.5 ml L⁻¹), panchagavya (30 ml L⁻¹), vermiwash (5 %), cow urine (5 %), Trichoderma harzianum (4 %), single cell protein (35 ml L⁻¹), seaweed extract (10 ml L⁻¹), GA₃ (100 ppm), KCl (1.0 %), KNO₃ (0.1 %), CaCl₂ (2 %) and salicylic acid (100 ppm), laid out in a Completely Randomized Design with four replications. Seeds were primed at 1:2 seed to solution ratio for 8 hours. Priming treatments differed significantly for all the seed quality parameters. GA₃ @ 100 ppm recorded the highest germination (92.50%), shoot length (7.65 cm), root length (8.16 cm), seedling length (15.81 cm), seedling dry weight (9.08 mg), seedling vigour index-I (1462) and index-II (840) as against 82.50 %, 5.65 cm, 5.75 cm, 11.40 cm, 7.10 mg, 941 and 586, respectively, in the unprimed control. It was on par with panchagavya @ 30 ml L⁻¹, vermiwash @ 5 %, KNO₃ @ 0.1 %, humic acid @ 2.5 ml L⁻¹ and CaCl₂ @ 2 % for most of the parameters. Among the organics, panchagavya @ 30 ml L⁻¹ was the best, offering an eco-friendly alternative to hormonal and chemical priming for better seedling establishment in ajwain.