The widespread use of 2,4-dichlorophenoxyacetic acid (2,4-D) herbicides has raised concerns regarding their potential toxicological effects on non-target organisms. This study evaluated the impact of 2,4-D exposure on biochemical, and electrolyte parameters in New Zealand rabbits following a 21-day exposure period. Forty rabbits were randomly assigned to three treatment groups and administered 3.00, 5.00, and 7.00 mg/L of 2,4-D, respectively. Blood samples were collected at the end of the experiment for analysis of serum enzymes, and electrolytes using standard laboratory procedures. Data were analyzed using one-way analysis of variance (ANOVA), and means were separated using Tukey's HSD test at p < 0.05. Results shows that serum enzymes evaluation were dose-dependent and recorded significant changes in aspartate aminotransferase, alanine aminotransferase, acid phosphatase. Serum electrolyte analysis demonstrated significant disturbances in potassium, chloride, calcium, and magnesium levels, particularly at higher concentrations of 2, 4-D exposure. The findings indicate that prolonged exposure to 2,4-D can induce biochemical, and electrolyte imbalances in New Zealand rabbits, suggesting potential adverse effects on blood physiology, organ function, and metabolic homeostasis. These results highlight the need for careful handling and monitoring of 2,4-D usage to minimize risks to animal health.