Even though the climatic conditions of the state Meghalaya of North East India favours wealth of fruit production, the state has the lowest development indices in production and marketing of fruits. The study on participatory agro-ecosystem assessment with application of GIS for development of model fruit village was conducted during February, 2020 at Nongpoh village, Ri-bhoi district of Meghalaya by the College of P.G. Studies in Agricultural Sciences (CPGS-AS), Central Agricultural University (Imphal), Umiam, Ri-bhoi Meghalaya. The study reported that 50000 m2, 25000 m2, 3000 m2, 400 m, 1000 m, 10500 m2, 6500 m2, 500 m and 480 m were the units of records as reported by the respondents of the study through resource mapping spatial PRA with respect to the following criteria (1) Consolidated Area under MFV, (2) Cultivable Area, (3) Area under water bodies, (4) Length of Stream available in the MFV, (5) Perimeter of MFV, (6) Area of Hillock #1, (7) Area of Hillock #2, (8) Altitude of Hillock #1, and (9) Altitude of Hillock #2. However, the 56431 m2, 29178 m2, 3656 m2, 835 m, 1184 m, 12600 m2, 6379 m2, 550 m and 545 m were the respective units of the same criteria measured by using Google-Earth Pro in ArcGIS 10.2 platform. On testing validity of the two approaches by employing Wilcoxon Signed Ranks Test, it was found that thee remained a significantly valid Difference at p = 0.021 with Wilcoxon T = 3.00 and n = 9.