Effect of benzophenone-3 at the environmentally relevant concentration on the liver of Zebra fish (Danio rerio (Hamilton)
S Velanganni, S Miltonprabu
The widespread use of Benzophenone-3 (BP-3) has resulted in its release into the ecosystem, and its potential impact on the aquatic ecosystem is of rising concern. Therefore, the current investigation was conducted to analyze the effect of BP-3 at environmentally relevant concentration (44 μg/L in river) in the zebra fish liver through the biochemical markers (protein, glycogen and lipids), hepatic markers, oxidative stress markers and histopathological analysis. The zebra fish was exposure to BP-3 at environmentally relevant concentration for 45 days. During different interval viz. 15, 30 and 45 days, all the above-mentioned markers in the liver and it’s histology were analyzed. The biochemical parameters like glycogen, protein triglyceride content were significantly declined with increased total cholesterol level in the zebra fish liver. The activity of hepatic markers like Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST), lipid peroxidation markers viz. thiobarbituric acid reactive substances (TBARS) and Hydrogen peroxide (H2O2) were found to be significantly higher meanwhile the activity of antioxidant superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx) and Glutathione (GSH) activity were found to be as a significant reduction in the liver of BP-3 treated fish for 30 and 45 days. Furthermore, the liver histology also showed abnormal changes in their morphology when compared to control. At the duration of 15 days, BP-3 exposure slightly altered all the parameters, but the values were non-significant when compared to control fish. From these results, we conclude that the treatment of BP-3 at environmentally relevant concentration can alter the physiology of the zebra fish liver.
S Velanganni, S Miltonprabu. Effect of benzophenone-3 at the environmentally relevant concentration on the liver of Zebra fish (Danio rerio (Hamilton). International Journal of Ecology and Environmental Sciences. 2020; 2(4): 640-646.