Optimization of fermentation condition for pectinase production from Aspergillus flavus, using African star cherry pectin as a carbon source
Nsude CA, Ujah II, Achikanu CE, Ezenwali MO, Udaya CL, Chilaka FC
Pectinases are heterogenous group of enzymes which are used to hydrolyze pectic substances and thus have become of high interest in many industries such as paper, textile and food industries. This research was aimed at determining the optimal submerged fermentation conditions for producing pectinase by Aspergillus flavus using African star cherry pectin as a carbon source. The design of this work involves extraction of pectin from ground African star cherry peels, isolation of pectin-hydrolyzing Aspergillus flavus and the optimization of biochemical parameters such as effects of pH, time, temperature, nitrogen source and pectin concentration for production of pectinolytic enzyme under best conditions. African star cherry pectin extraction yield was calculated to be 8.5%. Aspergillus flavus isolate was screened for pectin depolymerizing ability using agar well diffusion assay and the result indicated a clearance zone of 16mM (1.6cm). Further studies on the effects of time, pH, temperature and nitrogen sources showed day 4, 5.5, 300C and Ammonium Sulphate optimal for pectinase production under submerged fermentation technique.